Journal: Journal of Cellular and Molecular Medicine

Article Title: Daqinjiao Decoction Ameliorates CSVD via RXR‐γ/PPAR‐γ/VEGF‐α Pathway: Insights From Transcriptome Sequencing and Network Pharmacology

doi: 10.1111/jcmm.70712

Figure Lengend Snippet: DQJD reduces CCH‐induced neuronal death and inflammation levels in vivo. (A) HE staining of cortex (scale bar = 100 μm) and hippocampal CA1 (scale bar = 100 μm). (B) The number of neurons in the cortex and hippocampal CA1 was evaluated by Image J software ( n = 3). (C) Serum levels of TNF‐α, COX2, and ICAM‐1 were measured by ELISA (mean ± SD, n = 8). ** p < 0.01 (versus control); ## p < 0.01 (versus model).

Article Snippet: Rat ELISA kits of TNF‐α (Lot.EK382/3), COX‐2 (Lot.CSB‐E13399r), and ICAM‐1 (Lot.PI495) were purchased from Multi Sciences (Lianke) Biotech Co. Ltd. (Zhejiang, China), CUSABIO Co. Ltd. (Wuhan, China), and Beyotime Biotech Inc. (Shanghai, China), respectively.

Techniques: In Vivo, Staining, Software, Enzyme-linked Immunosorbent Assay, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Glutathione peroxidase 4 restrains temporomandibular joint osteoarthritis progression by inhibiting ferroptosis

doi: 10.1111/jcmm.18377

Figure Lengend Snippet: Ferroptosis was activated in TMJOA. (A) 8‐OHdG level in synovial fluid from TMJOA patients and normal patients (patients with temporomandibular fractures). (B) Representative histopathological haematoxylin–eosin, safranin O/fast green and alcian blue staining of undamaged and TMJ tissues from TMJOA patients. (C) Fe 2+ , Fe 3+ , and total iron levels were analysed in synovial tissues from TMJOA and normal patients. (D) Glutathione peroxidase activity levels were detected in synovial tissues from TMJOA and normal patients. (E) GSH contents and ratio of GSH/GSSG were measured in synovial tissues from TMJOA and normal patients. (F) The mRNA level of GPX4 was determined by qRT‐PCR assay in synovial tissues from TMJOA and normal patients. (G) The protein level of GPX4 was determined by western blot assay in synovial tissues from TMJOA and normal patients. ** p < 0.01; *** p < 0.001.

Article Snippet: The levels of Glutathione peroxidase, GSH/GSSG and MDA in the supernatant of lysed cells were assessed with a rat 8‐OHdG ELISA kit (ml028318, MLBIO, Shanghai, China), Glutathione peroxidase ELISA kit (S0056, Beyotime Biotechnology, Shanghai, China), GSH/GSSG ELISA kit (S0053, Beyotime Biotechnology, Shanghai, China) and MDA ELISA kit (S0131S, Beyotime Biotechnology, Shanghai, China) as instructions indicated.

Techniques: Staining, Activity Assay, Quantitative RT-PCR, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Glutathione peroxidase 4 restrains temporomandibular joint osteoarthritis progression by inhibiting ferroptosis

doi: 10.1111/jcmm.18377

Figure Lengend Snippet: Ferroptosis was activated in the primary OA‐FLSs. (A) Fe 2+ , Fe 3+ , and total iron levels in N‐FLSs and OA‐FLSs. (B) Glutathione peroxidase activity levels were detected in N‐FLSs and OA‐FLSs. (C) GSH contents and ratio of GSH/GSSG were measured in N‐FLSs and OA‐FLSs. ** p < 0.01.

Article Snippet: The levels of Glutathione peroxidase, GSH/GSSG and MDA in the supernatant of lysed cells were assessed with a rat 8‐OHdG ELISA kit (ml028318, MLBIO, Shanghai, China), Glutathione peroxidase ELISA kit (S0056, Beyotime Biotechnology, Shanghai, China), GSH/GSSG ELISA kit (S0053, Beyotime Biotechnology, Shanghai, China) and MDA ELISA kit (S0131S, Beyotime Biotechnology, Shanghai, China) as instructions indicated.

Techniques: Activity Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Glutathione peroxidase 4 restrains temporomandibular joint osteoarthritis progression by inhibiting ferroptosis

doi: 10.1111/jcmm.18377

Figure Lengend Snippet: Ferroptosis inhibitors promote HFLSs survival by suppressing ferroptosis. (A) Cell viability was assessed by CCK‐8 assay in indicated groups. (B) The concentration of MDA was assessed by ELISA assay in indicated groups. (C) Glutathione peroxidase activity levels were detected in indicated groups. (D) GSH contents and ratio of GSH/GSSG were measured in indicated groups. (E) The protein levels of GPX4, ADAMTS5, SLC3A2, ACSL4, and GAPDH in indicated groups were determined by western blot assay. ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The levels of Glutathione peroxidase, GSH/GSSG and MDA in the supernatant of lysed cells were assessed with a rat 8‐OHdG ELISA kit (ml028318, MLBIO, Shanghai, China), Glutathione peroxidase ELISA kit (S0056, Beyotime Biotechnology, Shanghai, China), GSH/GSSG ELISA kit (S0053, Beyotime Biotechnology, Shanghai, China) and MDA ELISA kit (S0131S, Beyotime Biotechnology, Shanghai, China) as instructions indicated.

Techniques: CCK-8 Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Glutathione peroxidase 4 restrains temporomandibular joint osteoarthritis progression by inhibiting ferroptosis

doi: 10.1111/jcmm.18377

Figure Lengend Snippet: GPX4 knockdown inhibited HFLSs survival by activating ferroptosis. (A) The mRNA level of GPX4 in indicated groups was determined by qRT‐PCR assay. (B) The protein levels of GPX4, ADAMTS5, SLC3A2, ACSL4, and GAPDH in indicated groups were determined by western blot assay. (C) Cell viability was assessed by CCK‐8 assay in indicated groups. (D) Fe 2+ , Fe 3+ , and total iron level in indicated groups. (E) Glutathione peroxidase activity levels were detected in indicated groups. (F) GSH contents and ratio of GSH/GSSG were measured in indicated groups. (G) The concentration of MDA was assessed by ELISA assay in indicated groups. ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The levels of Glutathione peroxidase, GSH/GSSG and MDA in the supernatant of lysed cells were assessed with a rat 8‐OHdG ELISA kit (ml028318, MLBIO, Shanghai, China), Glutathione peroxidase ELISA kit (S0056, Beyotime Biotechnology, Shanghai, China), GSH/GSSG ELISA kit (S0053, Beyotime Biotechnology, Shanghai, China) and MDA ELISA kit (S0131S, Beyotime Biotechnology, Shanghai, China) as instructions indicated.

Techniques: Quantitative RT-PCR, Western Blot, CCK-8 Assay, Activity Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Glutathione peroxidase 4 restrains temporomandibular joint osteoarthritis progression by inhibiting ferroptosis

doi: 10.1111/jcmm.18377

Figure Lengend Snippet: GPX4 overexpression promoted HFLSs survival by inhibiting ferroptosis. (A) The protein levels of GPX4, ADAMTS5, SLC3A2, ACSL4, and GAPDH in indicated groups were determined by western blot assay. (B) Cell viability was assessed by CCK‐8 assay in indicated groups. (C) Fe 2+ , Fe 3+ , and total iron level in indicated groups. (D) Glutathione peroxidase activity levels were detected in indicated groups. (E) GSH contents and ratio of GSH/GSSG were measured in indicated groups. (F) The concentration of MDA was assessed by ELISA assay in indicated groups. ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The levels of Glutathione peroxidase, GSH/GSSG and MDA in the supernatant of lysed cells were assessed with a rat 8‐OHdG ELISA kit (ml028318, MLBIO, Shanghai, China), Glutathione peroxidase ELISA kit (S0056, Beyotime Biotechnology, Shanghai, China), GSH/GSSG ELISA kit (S0053, Beyotime Biotechnology, Shanghai, China) and MDA ELISA kit (S0131S, Beyotime Biotechnology, Shanghai, China) as instructions indicated.

Techniques: Over Expression, Western Blot, CCK-8 Assay, Activity Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Glutathione peroxidase 4 restrains temporomandibular joint osteoarthritis progression by inhibiting ferroptosis

doi: 10.1111/jcmm.18377

Figure Lengend Snippet: GPX4 Overexpression and Fer‐1 significantly improve the pathological changes in the TMJOA Rat Model. (A) The analgesic effect of celecoxib on chronic inflammation in rats was evaluated using the hot water tail‐flick test (HWT). (B) HE staining and Safranin O staining depicts the changes in cartilage structure and matrix proteoglycans of TMJ tissues. (C) Severity analysis of the TMJOA model using the OARSI scoring system. (D) Quantification of chondrocyte counts in the histological slices. (E) Statistical analysis of each group's percentage of proteoglycan area. (F–H) Assessment of iron concentration in various groups using an iron assay kit in TMJ synovial tissues. (I, J) The measurement of GSH contents and the ratio of GSH/GSSG in TMJ synovial tissues. (K, L) Immunohistochemistry (IHC) staining and evaluation of GPX4 expression intensity in TMJ tissues. Compared with Sham group, *** p < 0.001; Compared with TMJOA group, ## p < 0.01.

Article Snippet: The levels of Glutathione peroxidase, GSH/GSSG and MDA in the supernatant of lysed cells were assessed with a rat 8‐OHdG ELISA kit (ml028318, MLBIO, Shanghai, China), Glutathione peroxidase ELISA kit (S0056, Beyotime Biotechnology, Shanghai, China), GSH/GSSG ELISA kit (S0053, Beyotime Biotechnology, Shanghai, China) and MDA ELISA kit (S0131S, Beyotime Biotechnology, Shanghai, China) as instructions indicated.

Techniques: Over Expression, Tail Flick Test, Staining, Concentration Assay, Iron Assay, Immunohistochemistry, Expressing

Journal: PLoS ONE

Article Title: Mid-Gestational Gene Expression Profile in Placenta and Link to Pregnancy Complications

doi: 10.1371/journal.pone.0049248

Figure Lengend Snippet: Maternal and offspring characteristics of REPROMETA samples used in the study for RT-qPCR and ELISA experiments.

Article Snippet: STC1 protein expression was measured using DuoSet ELISA development kit (#DY2958, lot #1215530; R&D Systems Europe, Ltd., Abingdon, UK) following manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR